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Bosses are very strong monsters found in boss rooms at the end of a floor. The boss at the end of the floor must be defeated in order to advance to the next floor. In later chapters, bosses are able to spawn as normal enemies. This starts in the caves, where the Gurglings are often encountered. The boss for a particular floor is chosen from the pool of bosses for the environment of the floor. Bosses may also spawn as champions, which can change various attributes such as health, damage, and attack pattern. Upon defeat, most bosses will drop an item from the boss item pool.

Bosses boast additional resistances to status effects. For example, fear occurs less often and the Night Light is unable to slow them down.

Additionally, some floors may spawn a room with a Mini-Boss.

Chapter 1[ | ]

The Basement[ | ]

Boss Monstro Boss Gemini
Boss Larry Jr.
Monstro Gemini Larry Jr.
Boss Dingle
Boss Gurglings
Boss Steven
Dingle Gurglings Steven

The Cellar[ | ]

Boss Pin
Boss Widow
Boss Blighted Ovum
Pin Widow Blighted Ovum
Boss Fistula
Boss Gurdy Jr.
Boss The Haunt
Fistula Gurdy Jr. The Haunt

The Basement and The Cellar[ | ]

Boss The Duke of Flies
Boss Famine
Boss Rag Man
The Duke of Flies Famine Rag Man File:DLC Indicator.png
Boss Little Horn
Boss Dangle
Boss Turdlings
Little Horn File:DLC Indicator.png Dangle File:DLC Indicator.png Turdlings File:DLC Indicator.png

Burning Basement File:DLC Indicator.png[ | ]

Boss Rag Man
Boss Little Horn
Boss Dangle
Rag Man File:DLC Indicator.png Little Horn File:DLC Indicator.png Dangle File:DLC Indicator.png
Boss Turdlings
Turdlings File:DLC Indicator.png

Chapter 2[ | ]

The Caves[ | ]

Boss Chub
Boss Gurdy
Boss Mega Maw
Chub Gurdy Mega Maw
Boss Mega Fatty
Boss C.H.A.D.
Boss Rag Mega
Mega Fatty C.H.A.D. Rag Mega File:Afterbirth† Indicator.png

The Catacombs[ | ]

Boss The Hollow
Boss The Husk
Boss Dark One
The Hollow The Husk Dark One
Boss Polycephalus
Boss Carrion Queen
Boss The Wretched
Polycephalus Carrion Queen The Wretched

The Caves and The Catacombs[ | ]

Boss Peep
Boss Gurdy Jr.
Boss Pestilence
Peep Gurdy Jr. Pestilence
Boss The Frail
Boss The Stain
Boss The Forsaken
The Frail File:DLC Indicator.png The Stain File:DLC Indicator.png The Forsaken File:DLC Indicator.png
Boss Big Horn
Big Horn File:Afterbirth† Indicator.png

Double Trouble[ | ]

Boss Dingle
Boss The Duke of Flies
Boss Gemini
(Double Trouble)

Dingle

(Double Trouble)

The Duke of Flies

(Double Trouble)

Gemini

Boss Widow
Boss Pin
Boss Polycephalus
(Double Trouble)

Widow

(Double Trouble)

Pin

(Double Trouble)

Polycephalus

Boss Larry Jr.
Boss Blighted Ovum
Boss Monstro
(Double Trouble)

Larry Jr.

(Double Trouble)

Blighted Ovum

(Double Trouble)

Monstro

Flooded Caves File:DLC Indicator.png[ | ]

Boss The Frail
Boss The Stain
Boss The Forsaken
The Frail File:DLC Indicator.png The Stain File:DLC Indicator.png The Forsaken File:DLC Indicator.png
Boss Big Horn
Big Horn File:Afterbirth† Indicator.png

Chapter 3[ | ]

Floor 1[ | ]

The Depths[ | ]

Boss The Cage
Boss Monstro II
Boss The Gate
Boss Gish
The Cage Monstro II The Gate Gish

Necropolis[ | ]

Boss The Adversary
Boss The Bloat
Boss Mask of Infamy
The Adversary The Bloat Mask of Infamy


The Depths and Necropolis[ | ]

Boss Loki
Boss War
Boss Brownie
Loki War Brownie File:DLC Indicator.png
Boss Sisters Vis
Sisters Vis File:Afterbirth† Indicator.png

Double Trouble[ | ]

Boss Gurdy Jr.
The Haunt
Boss The Wretched
Boss The Husk
(Double Trouble)

Gurdy Jr.

(Double Trouble)

The Haunt

(Double Trouble)

The Wretched

(Double Trouble)

The Husk

Boss Peep
Boss Mega Fatty
Boss Dark One
(Double Trouble)

Peep

(Double Trouble)

Mega Fatty

(Double Trouble)

Dark One

Boss Mega Maw
Boss Larry Jr.
Boss Blighted Ovum
(Double Trouble)

Mega Maw

(Double Trouble)

Larry Jr.

(Double Trouble)

Blighted Ovum

Dank Depths File:DLC Indicator.png[ | ]

Boss Brownie
Brownie File:DLC Indicator.png

Floor 2[ | ]

The Depths, Necropolis and Dank Depths[ | ]

Boss Mom
Mom

Chapter 4[ | ]

Floor 1[ | ]

The Womb[ | ]

Boss Mr. Fred
Boss Scolex
Mr. Fred Scolex

Utero[ | ]

Boss Mama Gurdy
Boss Lokii
Mama Gurdy Lokii

The Womb and Utero[ | ]

Boss Blastocyst
Boss The Bloat
Boss Daddy Long Legs
Blastocyst The Bloat Daddy Long Legs
Boss Teratoma
Boss Triachnid
Boss Death
Teratoma Triachnid Death
Boss Conquest
Conquest

Double Trouble[ | ]

Boss The Cage
Boss Gurdy
Boss The Fallen
Boss The Gate
Boss Loki
(Double Trouble)

The Cage

(Double Trouble)

Gurdy

(Double Trouble)

The Fallen

(Double Trouble)

The Gate

(Double Trouble)

Loki

Scarred Womb[ | ]

Floor 2[ | ]

The Womb, Utero and Scarred Womb[ | ]

Boss Mom's Heart
Boss It Lives
Mom's Heart It Lives

Blue Womb File:DLC Indicator.png[ | ]

Boss Hush
Hush File:DLC Indicator.png


The Void File:Afterbirth† Indicator.png[ | ]

Boss Delirium
Delirium File:Afterbirth† Indicator.png

Chapter 5[ | ]

Sheol[ | ]

Boss Satan
Satan

The Cathedral[ | ]

Isaac

Chapter 6[ | ]

The Chest[ | ]

???
???

Dark Room[ | ]

Boss The Lamb
The Lamb

Golden Door Boss[ | ]

Boss Mega Satan
Mega Satan

Universal[ | ]

Universal Bosses are those which may appear at the end of floors 1-5 and 7, replacing a boss from the pool for that environment.

Boss The Fallen
Boss The Headless Horseman
The Fallen The Headless Horseman

Devil and Angel Room[ | ]

Devil Room[ | ]

Boss Krampus
Krampus

Angel Room[ | ]

Boss Angel
Boss Angel2
Uriel Gabriel

Seven Deadly Sins and Others[ | ]

Seven Deadly Sins[ | ]

The Seven Deadly Sins are mini-bosses that can be encountered on any floor, and will appear on post-Womb/Utero floors as regular enemies. Unlike with regular bosses, there is no special door leading to a mini-boss room, meaning that there is no way to tell whether a room is inhabited by a mini-boss unless the player is in possession of an item that reveals map icons such as Compass or a Map item. Once in a mini-boss room, the player cannot escape through means of bombing the door, as it will be boarded off.

Each of the Sins has specific drops, that are consumables or a pedestal item (less frequently).
In a room containing multiple Sins, only the last one will drop something, allowing you to choose what drop you want by killing a certain mini-boss last.

All them are based on the Seven Deadly Sins.

Boss Gluttony
Boss Wrath
Envy Gluttony Wrath
Boss Pride
Boss Lust
Boss Greed
Pride Lust Greed
Boss Sloth
Sloth

These monsters can also spawn as much stronger alternatives, known as the Seven Super Deadly Sins.

Boss Super Envy
Boss Super Gluttony
Boss Super Wrath
Super Envy Super Gluttony Super Wrath
Boss Super Pride
Boss Super Lust
Boss Super Greed
Super Pride Super Lust Super Greed
Boss Super Sloth
Super Sloth

There is also an Ultra variant of both Pride and Greed. Ultra Pride resembles Edmund and Florian, while Ultra Greed serves as the final boss for Greed Mode.

Boss Ultra Pride
Ultra Pride

Greed Mode File:DLC Indicator.png[ | ]

Boss Ultra Greed
Ultra Greed File:DLC Indicator.png

All Mini-Bosses[ | ]

The Seven Deadly Sins[ | ]

Boss Gluttony
Boss Wrath
Envy Gluttony Wrath
Boss Pride
Boss Lust
Boss Greed
Pride Lust Greed
Boss Sloth
Sloth

The Seven Super Deadly Sins[ | ]

Boss Super Envy
Boss Super Gluttony
Boss Super Wrath
Super Envy Super Gluttony Super Wrath
Boss Super Pride
Boss Super Lust
Boss Super Greed
Super Pride Super Lust Super Greed
Boss Super Sloth
Super Sloth

Ultra Pride[ | ]

Boss Ultra Pride
Ultra Pride

Krampus[ | ]

Boss Krampus
Krampus

Angels[ | ]

Boss Angel
Boss Angel2
Uriel Gabriel

All Bosses[ | ]

Boss Monstro Boss Gemini
Boss Larry Jr.
Monstro Gemini Larry Jr.
Boss Dingle
Boss Gurglings
Boss Steven
Dingle Gurglings Steven
Boss Pin
Boss Widow
Boss Blighted Ovum
Pin Widow Blighted Ovum
Boss Fistula
Boss Gurdy Jr.
Boss The Haunt
Fistula Gurdy Jr. The Haunt
Boss The Duke of Flies
Boss Rag Man
Boss Little Horn
The Duke of Flies Rag Man File:DLC Indicator.png Little Horn File:DLC Indicator.png
Boss Dangle
Boss Turdlings
Boss Chub
Dangle File:DLC Indicator.png Turdlings File:DLC Indicator.png Chub
Boss Gurdy
Boss Mega Maw
Boss C.H.A.D.
Gurdy Mega Maw C.H.A.D.
Boss Mega Fatty
Boss The Hollow
Boss The Husk
Mega Fatty The Hollow The Husk
Boss Dark One
Boss Polycephalus
Boss The Frail
Dark One Polycephalus The Frail File:DLC Indicator.png
Boss The Stain
Boss The Forsaken
Boss Carrion Queen
The Stain File:DLC Indicator.png The Forsaken File:DLC Indicator.png Carrion Queen
Boss The Wretched
Boss Peep
Boss The Cage
The Wretched Peep The Cage
Boss Monstro II
Boss The Gate
Boss Gish
Monstro II The Gate Gish
Boss The Adversary
Boss The Bloat
Boss Mask of Infamy
The Adversary The Bloat Mask of Infamy
Boss Loki
Boss Brownie
Boss Mr. Fred
Loki Brownie File:DLC Indicator.png Mr. Fred
Boss Scolex
Boss Mama Gurdy
Boss Lokii
Scolex Mama Gurdy Lokii
Boss Blastocyst
Boss Daddy Long Legs
Boss Teratoma
Blastocyst Daddy Long Legs Teratoma
Boss Triachnid
Boss The Fallen
Triachnid The Fallen

The Harbingers[ | ]

Boss Famine
Boss Pestilence
Boss War
Famine Pestilence War
Boss Death
Boss Conquest
Boss The Headless Horseman
Death Conquest The Headless Horseman

Final Bosses[ | ]

Boss Mom
Boss Mom's Heart
Boss It Lives
Boss Hush
Boss Delirium
Mom Mom's Heart It Lives Hush File:DLC Indicator.png Delirium File:Afterbirth† Indicator.png
Boss Satan
Boss The Lamb
???
Satan The Lamb Isaac ???
Boss Mega Satan
Boss Ultra Greed
Boss Ultra Greedier
Mega Satan Ultra Greed File:DLC Indicator.png Ultra Greedier File:Afterbirth† Indicator.png

Trivia[ | ]

  • "Originally every boss/enemy in Isaac was going to resemble Isaac in some way. this is the case for most."[1]
  • Double Trouble boss pools can have bosses from any of the previous chapters' pools, and are the same for both versions of that chapter's floor. This means that, for example, Double Trouble boss fights in the Caves can feature bosses from the Cellar or the Basement, and every Double Trouble fight in the Caves can also be encountered in the Catacombs.
  • Bosses often come in three versions: normal (For example, Monstro), posthumous (Monstro II), and alternate (Gish). Usually, the posthumous boss looks like the original with some horrible mutilation; for example, Monstro and Chub had most of their skin ripped off.

Bugs[ | ]

Bug Bug! Defeating a boss quickly enough might cause the boss music to play, even after the boss dies.

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